Table 1 PCR Round 1 Master Mix Specifics

From: Potent AMA1-specific human monoclonal antibody against Plasmodium vivax Pre-erythrocytic and Blood Stages

Component

Volume for 1 well (μL)

RNAse Free Water

10.79

5x Q-Solution

4.0

10x HotStar Taq PCR Buffer

2.0

25 mM MgCl2

0.55

dNTP Mix (25 mM each dNTP)

0.20

5’ LV-Primer Group Mix

0.130

3’ Primer (50 mM)

0.130

HotStar Taq (5 units/μL)

0.20

DNA Template

2.00

Total Volume (w/out DNA)

18.00

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