Table 4 Specific V(D)J Region Amplification Master Mix Specifics

From: Potent AMA1-specific human monoclonal antibody against Plasmodium vivax Pre-erythrocytic and Blood Stages

Component

Volume for 1 well (μL)

RNAse Free Water

18.0

5x Q-Solution

8.0

10x HotStar Taq PCR Buffer

4.0

25 mM MgCl2

1.0

dNTP Mix (25 mM each dNTP)

0.4

5’ primer at 5 μm

4.0

3’ primer at 5 μm

4.0

HotStar Taq (5 units/μL)

0.4

DNA Template

4.2

Total Volume (w/out DNA)

39.8

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