Fig. 4: Genomic signatures in the subdivision of five metastatic state trajectories.

a UMAP plot of five metastatic state trajectories, in addition, cells were re-clustered into 22 clusters. b Cellular proportions of five metastatic states in 22 cell clusters. c Cell numbers of five metastatic states in 22 cell clusters. d Pie charts illustrating the distributions of cell clusters in five different organ metastases. e Genomic signatures of top gene expression in 22 cell clusters. f The dominant cell clusters in each distant metastasis were chosen for differential expression analysis. C4 and C12 were analyzed due to their predominance in bone metastasis ((1) and (2)). C9 cluster was enriched both in "Brainplus" (brain metastasis with concurrent metastasis) and "Boneplus" (bone metastasis with concurrent metastasis); considering its multiple metastasis signatures, comparative analyses were conducted on both "Brainplus" and "Boneplus" ((3) and (4)). C0 cluster was enriched both in the lung and pleural metastases, and subsequent comparative analyses were conducted ((5) and (6)). Source data are provided as a Source Data file.