Fig. 4: The conserved transcription factor TRA-1 mediates environmental sex determination. | Nature Communications

Fig. 4: The conserved transcription factor TRA-1 mediates environmental sex determination.

From: Rapid chromosome evolution and acquisition of thermosensitive stochastic sex determination in nematode androdioecious hermaphrodites

Fig. 4

a Orthologs of C. elegans genes involved in sex determination in Pristionchus and outgroup nematodes. The colour panels indicate orthology relationship. Genes positioned more to the right are upstream in the pathway. The species phylogeny is presented on the right. b Establishment of CRISPR engineering in a nematode system, P. mayeri. The genomic Region of Pmay-tra-1.1 is displayed. Exons are represented by black boxes. A guide RNA was designed within the conserved zinc-finger domain. Two mutants with 2-bp deletion (tu1863) and 5-bp insertion (tu1864) were isolated. Phenotypes of homozygous Pmay-tra-1.1 mutants in whole body (c) and posterior male-specific organs (d, e). Scale bars indicate 50 µm (c) and 20 µm (d, e). The present individual was one of ten tested male-like individuals, all of which indicated the same morphology. f Proportion of different sexes in Pmay-tra-1.1 mutants. The number displayed in the centre of each column represents sample size. g Percentage of successful crosses using Pmay-tra-1.1 mutant males.

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