Fig. 6: Independent evolution of stochastic sex determination in P. entomophagus.
a No coverage difference across the entire genome was observed between sexes in P. entomophagus. The average log2 coverage of 100-kb sliding windows was normalised for each individual and the median of the normalised coverage are presented as a line. The area between the first and third quartiles is shown in the light-coloured ribbon. Coloured bars on the axes corresponds to NigonE (orange), NigonN (purple) and NigonX (green). b Test of temperature effects on the percentage of males in P. entomophagus (N = 10 each). Each jitter point represents the male ratio of a single biological replicate, with the red bar indicating the average across replications. A statistic test was conducted with GLMM for pairwise comparison. The effect of temperature differences on the log odd (and its 95% confidence interval) was estimated as −1.07 ([−1.85, −0.462]). The χ2 statistics and p-value are 9.59 and 1.96 × 10−3, respectively. The degree of freedom is 1. The p-value is one-sided due to goodness-of-fit test and represented as stars (*p < 0.05, **p < 0.01, ***p < 0.001).
