Fig. 5: Mollicutes in the mesoglea surrounding the axis in Callogorgia delta. | Nature Communications

Fig. 5: Mollicutes in the mesoglea surrounding the axis in Callogorgia delta.

From: Discovery of deep-sea coral symbionts from a novel clade of marine bacteria with severely reduced genomes

Fig. 5

a The orientation of polyps around the central axis shown in a thick section (500 nm) embedded in Spurr’s epoxy resin stained with toluidine blue and methylene blue. b, c Bacterial signals in mesoglea near the axis in four of five colonies from tissue sections hybridized using CARD-FISH with the general eubacterial probe mix (EUB338 I-III, green) and stained with DAPI (blue). d Adjacent section to (c) hybridized with negative control probe showing DAPI fluorescence overlapping region where signal was detected. e–g Transmission electron micrographs showing bacteria with characteristic mollicutes appearance seen in five of six colonies. Unlabeled arrows indicate bacteria. Boxes indicate the exact region or type of region next image shows. ax central axis, sl sclerite lacuna, sc stem canal, gc granular cell, mg mesoglea, cf collagen fiber.

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