Fig. 1: The vH discriminates contexts following social reward contextual conditioning.
From: Hippocampal contextualization of social rewards in mice
a Schematic and image of vH tetrode microdrives. b Experimental design of social reward (SR) CPP task with representative trajectories. c Context discrimination score per session. Repeated measures (RM) one-way ANOVA F(2.8,61.8) = 18.55, P = 2.1 × 10−8. Pre-Train vs. Train-1 P = 7.9 × 10−7, Train-2 P = 2.9 × 10−7, Train-3 P = 1.9 × 10−6, Pre- vs. Post-Train P = 5.3 × 10−6. One-sample t-test vs. zero, Pre-Train t(22) = 5.19 P = 1.7 × 10−5, Train-1 t(22) = 4.1 P = 0.0006, Train-2 t(22) = 5.22 P = 1.5 × 10−5, Train-3 t(22) = 2.8 P = 0.0104, Post-Train t(22) = 1.7 P = 0.103. d Firing rate of two SR-context neurons at Post-Train. e Normalized firing rate of context neurons at Pre- and Post-Train. Two-way RM-ANOVA, SR-context neurons: session × context, F(1,73) = 22.52, P = 0.00001; SR-context Pre- vs. Post-Train P = 1.64 × 10−11; Post-Train SR- vs. neutral-context P = 4.07 × 10−8, n = 74 neurons. One-sample t-test, Pre-Train SR-context t(73) = 2.34 P = 0.022; Post-train SR-context t(73) = 4.45 P = 0.00003. Two-way RM-ANOVA, neutral-context neurons: context, F(1,46) = 19.78 P = 0.000055; Post-Train SR- vs. neutral-context P = 5.11 × 10−6; neutral-context Pre- vs. Post-Train P = 0.0072, n = 47 neurons. f Normalized firing rate of SR- and neutral-context neurons upon entry into context-B or -A at Pre-Train: context-B, −2 to −1s, neutral-context neurons, t(45) = 2.11 P = 0.041; SR-context neurons, t(67) = 2.46 P = 0.017, and during (g) Post-Train: three-way Mixed-ANOVA, pre-entry group × context × time, F(3.7, 448.5) = 3.94 P = 0.005; post-entry group x context: F(1,120) = 12.65 P = 0.0005. One-sample t-test, pre-entry into neutral-context, neutral-context neurons: −2 to −1 s, t(47) = 3.01 P = 0.004; −1 to 0 s, t(47) = 2.19 P = 0.034; pre-entry into SR-context, SR-context neurons: −2 to −1 s, t(73) = 0.89 P = 0.38; −1 to 0 s, t(73) = 2.39 P = 0.02. h Correlation between context discrimination and proportion of SR-context neurons during Post-Train. Pearson’s r (2-tailed), r = 0.4 P = 0.0141. All multiple-comparisons were Bonferroni-corrected 2-tailed t-tests. Data are from n = 23 mice. Bar charts are presented as mean ± SEM.
