Fig. 4: Ventral hippocampal neurons reorganize their context activity upon social reward contextual learning.
From: Hippocampal contextualization of social rewards in mice
a Percentages of context neurons at Pre- and Post-Train. Chi-squared test, χ2(2) = 11.26 P = 0.0036, n = 23 mice. b Contingency matrix of neuron percentages and counts per context category at Pre- and Post-Train. χ2(4) = 9.75, P = 0.0448, n = 23 mice. c Schematic and example image of optogenetic strategy. d Schematic of SR-CPP task with laser illumination in SR-context during training. (C-F) n = 6 EYFP, n = 7 eArch3.0. e Behavioural discrimination score during training. Two-way Mixed-ANOVA, group effect F(1,6) = 7.617 P = 0.0329; f Discrimination score during Pre- and Post-Train. Two-way Mixed-ANOVA, group × session F(1,4) = 40.98, P = 0.0031. Bonferroni-corrected comparisons, Post-Train EYFP vs. eArch3.0, P = 0.0048. g Schematic of calcium imaging strategy. Inset, processed 1-photon Ca2+ signals in vH pyramidal neurons. (G-N) tdTomato: n = 120 neurons, 2 mice; eNpHR3.0: n = 273 neurons, 3 mice. h Representative Ca2+ traces of SR-context neurons during SR-CPP at Post-Train. i Percentages of context neurons during Pre- and Post-train. tdTomato: χ2(2) = 6.78 P = 0.0337; eNpHR3.0: χ2(2) = 0.94 P = 0.625. j Contingency table of neurons in each context category at Pre-Train and percentage at Post-Train. Numbers indicate percentages of neurons. Non-preferring at Pre-train: χ2(2) = 8.15 P = 0.017; SR-preferring at Pre-train: χ2(2) = 2.67 P = 0.263; neutral-preferring at Pre-train: χ2(2) = 0.92 P = 0.631. k Z-scored Ca2+ activity of SR-context neurons upon entry into SR-context at Post-Train. Two-way Mixed-ANOVA, pre-entry group × time F(4.6,644.8) = 8.27 P = 3.97 × 10−7; post-entry group F(1,141) = 49.52 P = 7.87 × 10−11. l Z-scored Ca2+ activity of neutral-context neurons upon entry into the neutral context at Post-Train. Two-way Mixed-ANOVA, pre-entry group × time: F(3.95,438.7) = 3.48 P = 0.008; post-entry group: F(1,111) = 16.19 P = 0.00011. Data are presented as the mean ± SEM.
