Fig. 2: p14^ARF engages in intra- and intermolecular contacts within the condensed phase with NPM1.

A A CC-DARR spectrum acquired for [¹³C,¹⁵N]-p14^ARF with 20 ms DARR mixing time shows resonances for T8 in two states, including in an expanded p14^ARF conformation (top), and in a collapsed p14^ARF conformation (bottom). B A CC-DARR spectrum acquired with 400 ms DARR mixing time shows additional cross-peaks indicating intramolecular contacts between T8 and H26. C The 2D-NHHC spectrum (gray) of p14^ARF (equal mixture of [¹³C]-p14^ARF and [¹⁵N]-p14^ARF) shows that sidechains within the p14^ARF N-terminus make intermolecular contacts within the condensed phase with NPM1. HNCA (magenta) and 2D-HNCACX (blue) spectra for [¹³C,¹⁵N]-p14^ARF are shown for reference. D Schematic describing possible modes of p14^ARF intra- and intermolecular interaction.