Fig. 9: Tumor suppression and immune activation in bilateral tumor model.

a Schematic illustration of the treatment procedure and assessment of therapeutic efficacy of BC@Z-M in bilateral tumor model. Created in BioRender. Li, W. (2024) BioRender.com/v06y872. b Tumor growth curve and (d) tumor weights in “PBS + L” or “BC@Z-M + L” group (n = 5 mice). c Photographs of the tumors resected from bilateral tumor-bearing mice after the treatment of “PBS + L” or “BC@Z-M + L” (n = 5 mice). e Representative flow cytometric analysis and (f) quantitative data of tumor-infiltrating CD8⁺CD3⁺ T and CD4⁺CD3⁺ T cells in primary and distant tumors from bilateral 4T1 tumor-bearing mice after “PBS + L” or “BC@Z-M + L” treatments (n = 4 mice). g Representative flow cytometric analysis and (h) quantitative data of tumor-infiltrating CD4⁺Foxp3⁺ Tregs in primary and distant tumors collected from bilateral 4T1 tumor-bearing mice after “PBS + L” or “BC@Z-M + L” treatments (n = 4 mice). i Representative flow cytometric analysis and (j) quantitative data of CD3⁺CD8⁺CD62L⁻CD44⁺ T_EM in spleen collected from bilateral 4T1 tumor-bearing mice in “PBS + L” or “BC@Z-M + L” group (n = 4 mice). All data are represented as mean ± SD. Statistical significance was determined using two-tailed Student’s t-test. Source data are provided as a Source Data file.