Fig. 4: Despite distinct activation patterns neoTCR-tg T cells demonstrate entity-independent, comparable in vivo tumor rejection upon first tumor encounter. | Nature Communications

Fig. 4: Despite distinct activation patterns neoTCR-tg T cells demonstrate entity-independent, comparable in vivo tumor rejection upon first tumor encounter.

From: High-resolution profile of neoantigen-specific TCR activation links moderate stimulation to increased resilience of engineered TCR-T cells

Fig. 4

a Schematic setting of xenograft first-encounter tumor rejection experiment with newly transduced neoTCR-tg T cells. b Tumor growth kinetics in a lymphoma model are displayed as tumor area (in cm2) for U698M-mut-mg-tumor-bearing NSG-mice comparing neoTCR-tg T cells to irrelevant TCR 2.5D6 until day 20. 30 × 106 TCR-tg T cells were injected per mouse. Mean and SD display rejection dynamics of tumor-bearing mice as biological replicates (n = 6). Parts of this dataset were published before31. Here, tumor rejection kinetics of KIF-sc1 and -sc2 analyzed in the same experiment are additionally shown. c Kaplan–Meier-survival curve is displayed for U698M-tumor-bearing mice injected with 30 × 106 neoTCR-tg T cells (Mantel–Cox test). d Tumor growth kinetics for U698M-mut-mg-tumor-bearing NSG-mice for KIF-P2, -sc1 and 2.5D6 after injection of 5 × 106 TCR-tg T cells per mouse (n = 4 biological replicates). e Kaplan–Meier-survival curve displayed for U698M-tumor-bearing mice injected with 5 × 106 neoTCR-tg T cells (Mantel–Cox test; KIF-P2 and KIF-sc1 p = 0.0067). f Tumor growth kinetics in a melanoma model for mut-mg-A2058-tumor-bearing NSG-mice after injection of 5 × 106 TCR-tg T cells per mouse (n = 5 biological replicates). g Kaplan–Meier-survival curve displayed for A2058-tumor-bearing mice injected with 5 × 106 neoTCR-tg T cells (Mantel–Cox test, KIF-P2 and KIF-sc1 p = 0.0026). For d–g mean and SEM display rejection dynamics.

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