Fig. 4: MenT3 specifically targets tRNASer in M. tuberculosis.
a Experimental conditions for tRNA-seq in M. tuberculosis. M. tuberculosis (Mtb) wild-type H37Rv strain and its isogenic mutant ∆menAT3 expressing MenT3 from the integrative pGMC vector were individually grown at 37 °C in 7H9 medium supplemented with 10% albumin-dextrose-catalase (ADC, Difco) and 0.05% Tween 80. When the OD600 reached to about 0.5, the anhydrotetracycline inducer (Atc, 200 ng ml−1) was added, and cells were collected after 0, 3, or 24 h incubation at 37 °C. Total RNA was extracted, and tRNA-seq was performed. b Percentage of modified tRNA per tRNA species identified for the mutant overexpressing MenT3 and panel c for the wild type strain. The names of the identified tRNA for both strains are shown on the left of (b). The data were presented as the mean value obtained from three independent experiments. A detailed view of the different modifications obtained for tRNASer is shown in Supplementary Fig. S3. Source data are provided as a Source Data file.
