Fig. 4: Catalase activity deficiency sensitizes Mtb to DNA damage.

a Expression of several DNA repair enzymes is higher in BDQ-treated ∆katG cells relative to BDQ-treated H37Rv cells as measured by RNA sequencing. alkA, radA, recG, and ung are members of gene Cluster 7. Expression data reported as smooth quantile normalized log₂ sequencing counts. n = 3 biological replicates. b ∆katG cells are sensitized to the DNA damaging agent phleomycin relative to wild-type cells in 8-day growth inhibition dose-response experiments. n = 3 biological replicates. c KatG-deficient mc²8245 cells are sensitized to phleomycin in 8-day dose-response experiments. n = 3 biological replicates. d INH-resistant clinical isolates are sensitized to phleomycin relative to INH-susceptible clinical isolates in 8-day dose-response experiments. n = 4 biological replicates for non-MDR INH-resistant TDR-TB-42 (red). n = 1 biological replicate for all other TDR-TB clinical strains. Error bars for INH-susceptible and MDR clinical strains computed across INH-susceptible and MDR clinical strains, respectively. Brown-Forsythe and Welch ANOVA tests were performed on RNA expression data with comparisons between BDQ-treated and untreated cells wild-type and ∆katG cells or between untreated wild-type and ∆katG with Dunnett’s T3 multiple comparisons test FDR correction, as indicated. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. Data depicted as mean ± SEM. Source data are provided in the Source Data file.