Fig. 5: The DosP catalytic pocket.

a Bound c-di-GMP is shown as blue sticks. The guanine bases are labeled G1 and G2. The two magnesium ions are shown as green spheres and labeled Me1 and Me2. Metal-coordinating residues are depicted as cyan sticks. The rest of the EAL domain is depicted as a transparent cartoon. b Residues interacting with c-di-GMP are shown as magenta sticks. The rest of the EAL domain is depicted as transparent cartoon. c Bar graph showing the k_cat values determined from the turnover of 2 µM c-di-GMP at 25 °C by 10 nM aerobic DosP^WT, DosP^E584A, DosP^R588A, DosP^R588E, and DosP^Y784A. Value for DosP^WT was taken from Fig. 1g, values for mutants are the mean of two independent experiments. The buffer for the assays was 20 mM Tris-HCl, 2.5 mM MgCl₂, 2.0 mM DTT, pH 8.0. Residues whose substitutions were tested in c are highlighted with circles in a and b. Panels a and b generated with PyMOL. Source data are provided as a Source Data file.