Fig. 6: Efficient SAX-7 endocytosis in the epidermis relies on both EAT-17 and BICD-1.
From: Endocytosis restricts dendrite branching via removing ectopically localized branching ligands
a Confocal images showing the distribution of endogenous SAX-7::mCherry in early endosomes (labeled by GFP::RAB-5, YFP::2xFYVE or RME-8::GFP), late endosomes (LE) and lysosomes (labeled by GFP::RAB-7) and lysosomes (labeled by SCAV-3::GFP) in epidermal cells in the wildtype. White arrows: organelles positive for SAX-7::mCherry; Yellow arrows: organelles negative for SAX-7::mCherry. The image in the lower right corner was 2 times enlarged. Scale bar, 20 μm. b Quantification of the percentage of vesicles with SAX-7::mCherry in their lumen in wildtype animals. All values are presented as mean ± s.e.m. n = 20 animals were quantified for each column. c Confocal images showing the distribution of endogenous SAX-7::mCherry in endosomal compartments (labeled by YFP::2xFYVE) in epidermal cells in different strains. RAB-5 CA: constitutively active RAB-5. The image in the lower right corner was 2 times enlarged. Scale bar, 20 μm. d Quantification of the percentage of endosomes with SAX-7::mCherry in their lumen for each strain. All values are presented as mean ± s.e.m. ns: not significant. ****p < 0.0001 (one-sided ANOVA with the Tukey correction). n = 20 animals were quantified for each column. For (b–d) source data are provided as a Source Data file.
