Fig. 7: EAT-17 and BICD-1 function in the endocytosis pathway to downregulate the branching ligand SAX-7.
From: Endocytosis restricts dendrite branching via removing ectopically localized branching ligands
a Confocal images to show PVD dendrites in wildtype, eat-17(ok2983) and bicd-1(zac51) treated with either control (empty vector) RNAi, rab-5 RNAi, or dyn-1 RNAi, respectively. An integrated transgene wyIs592 [ser2prom3::myri-gfp] was used to label PVD dendrites. Scale bar, 20 μm. b Quantification of number of ectopic branches in the lateral regions of different strains showed in (a). All values are presented as mean ± s.e.m. ns: not significant (one-sided ANOVA with the Tukey correction). n = 20 animals were quantified for each column. c Confocal images to show SAX-7::GFP in wildtype, eat-17(ok2983) and bicd-1(zac51) treated with either control (empty vector) RNAi, rab-5 RNAi, or dyn-1 RNAi, respectively. wyIs50005 [Pdpy-7::sax-7::gfp + ser2prom3::myri-mcherry] was used to label SAX-7 in the epidermis. Asterisks: ectopic GFP expression in the spermatheca. Scale bar, 20 μm. d Quantifications of the relative fluorescence intensity of SAX-7::GFP in the lateral region in different strains showed in (c). All values are presented as mean ± s.e.m. WT L4440 RNAi: n = 33 animals; eat-17(ok2983) L4440 RNAi: n = 30 animals; bicd-1(zac51) L4440 RNAi: n = 31 animals; WT rab-5 RNAi: n = 28 animals; eat-17(ok2983) rab-5 RNAi: n = 32 animals; bicd-1(zac51) rab-5 RNAi: n = 30 animals; WT dyn-1 RNAi: n = 29 animals; eat-17(ok2983) dyn-1 RNAi: n = 37 animals; bicd-1(zac51) dyn-1 RNAi: n = 34 animals. ns: not significant. **p < 0.01 (one-sided ANOVA with the Tukey correction). n ≥ 25 animals were imaged and quantified for each column. e Confocal images of GFP::RAB-5 positive spots in the epidermis of different strains, including wildtype, eat-17(ok2983) and bicd-1(zac51) treated with control RNAi, chc-1 RNAi, dyn-1 RNAi, or dpy-23 RNAi respectively. f Quantification of number of GFP::RAB-5 positive vesicles in the strains showed in (e). All values are presented as mean ± s.e.m. WT L4440 RNAi: n = 25 animals; eat-17(ok2983) L4440 RNAi: n = 27 animals; bicd-1(zac51) L4440 RNAi: n = 27 animals; WT chc-1 RNAi: n = 27 animals; eat-17(ok2983) chc-1 RNAi: n = 27 animals; bicd-1(zac51) chc-1 RNAi: n = 27 animals; WT dyn-1 RNAi: n = 27 animals; eat-17(ok2983) dyn-1 RNAi: n = 27 animals; bicd-1(zac51) dyn-1 RNAi: n = 27 animals; WT dpy-23 RNAi: n = 29 animals; eat-17(ok2983) dpy-23 RNAi: n = 27 animals; bicd-1(zac51) dpy-23 RNAi: n = 27 animals. ns: not significant. ****p < 0.0001 (one-sided ANOVA with the Tukey correction). For (b–f) source data are provided as a Source Data file.
