Fig. 7: Transcriptomic Analysis in ADAR-KO Teratomas Reveals Germ-Layer Specific Fitness Defects.
From: Charting and probing the activity of ADARs in human development and cell-fate specification
a Workflow schematic for ADAR-KO screen in PGP1-Cas9 iPSCs, followed by teratoma formation and downstream transcriptomic analysis. b Pan-teratoma global transcriptomic variation across perturbation conditions. c Cell-type specific gene-module effect across perturbation conditions. d Differential expressed genes (DEGs) for ADAR-KO versus AAVS1-KO across the three germ layers. Teratoma cell types from the same germ-layer are transcriptomicaly grouped together. e Multi-lineage fitness analysis assessing the fitness defect across perturbation conditions. Cell counts from the same germ-layer are summed together. N = 4 unique teratomas used as independent biological replicates. Centerline of the box represents the mean, the box represents the 95% confidence interval, and the error bars are the standard error of the mean. (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, and ****p ≤ 0.0001; ns, not significant).
