Fig. 1: Enhanced transcription from H3K56ac-containing chromatin in vitro. | Nature Communications

Fig. 1: Enhanced transcription from H3K56ac-containing chromatin in vitro.

From: The BAF complex enhances transcription through interaction with H3K56ac in the histone globular domain

Fig. 1

p53- and p300-dependent transcription from recombinant chromatins containing full-length histone octamers harboring an individually acetylated lysine residue on H3 (a); full-length unmodified, H3K56ac and H3K56R histone octamers (b); and N-terminal tailless unmodified, H3K56ac, and H3K56R histone octamers (c), assembled using the ACF/NAP1 chromatin assembly system. d p53- and p300-dependent transcription from recombinant chromatins containing full-length unmodified, H3K56ac, and H3K56R histone octamers assembled using salt dialysis. Relative transcription levels were measured by autoradiography and normalized to those observed with the addition of p53 and p300 to chromatin containing unmodified histone octamers. Data are representative of at least two independent experiments. Source data are provided as a Source Data file.

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