Fig. 4: Enhanced nucleosome remodeling and transcriptional activity by the BAF complex in the presence of H3K56ac. | Nature Communications

Fig. 4: Enhanced nucleosome remodeling and transcriptional activity by the BAF complex in the presence of H3K56ac.

From: The BAF complex enhances transcription through interaction with H3K56ac in the histone globular domain

Fig. 4

a REA assay using mononucleosomes assembled with rhodamine-labeled 100N100 DNA and purified BAF complex. Left: Nucleosome-remodeling activity measured by fluorography. Note that the restriction enzyme, HhaI, was added to all reactions except lane 1. Right: Levels of remodeled nucleosomes were quantitated using ImageJ. Data are presented as mean values ± SD of three independent experiments. b REA assay with purified BAF complexes harboring a mutation in the DPF domain of DPF2. Data are presented as mean values ± SD of three independent experiments. P-values for the reactions with WT and R350H DPF2-containing complexes were determined by two-tailed unpaired Student’s t-test. c In vitro chromatin-transcription assay with purified BAF complexes containing WT or R350H DPF2. Note that p300 was added to all reactions. Relative transcription levels were measured by autoradiography and normalized to those observed with the addition of p53 and p300 to chromatin containing unmodified histone octamers (lane 2). Data are representative of two independent experiments. Source data are provided as a Source Data file.

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