Fig. 3: The effect of RapP on the expressions of sporulation genes.
From: Plasmid-encoded phosphatase RapP enhances cell growth in non-domesticated Bacillus subtilis strains
A Relative mRNA levels of four sporulation genes in DS7906 strain compared with DK1042 strain growing in mannose and ribose media, respectively. Data are presented as the mean values ± standard deviations (SD) of several biological replicates. n = 4 for all the conditions except in the case of sigE data on ribose, for which n = 5. B Growth-rate dependent promoter activities of spoIIE gene in DK1042 and DS7906 strains. Data are presented as the mean values ± standard deviations (SD) of several biological replicates. n = 3 and n = 4 for DK1042 and DS7906 strain, respectively. C Growth-rate dependent promoter activities of spoIIGA in DK1042 and DS7906 strains. Data are presented as the mean values ± standard deviations (SD) of four biological replicates (n = 4). D Growth-rate dependent promoter activities of spoIIE gene in wild type 168 and its spo0A-null strains. Data are presented as the mean values ± standard deviations (SD) of three biological replicates (n = 3). E Growth-rate dependent promoter activities of spoIIGA gene in wild type 168 and its spo0A-null strains. Data are presented as the mean values ± standard deviations (SD) of several biological replicates. n = 3 for all the data points. The LacZ reporter activities were measured by a fluorescent MUG substrate (see “Methods”) and expressed as relative fluorescence units (RFU). Source data are provided as a Source Data file.
