Fig. 2: NMJ analysis in co-cultures.
From: HuD impairs neuromuscular junctions and induces apoptosis in human iPSC and Drosophila ALS models
A Schematic representation of co-cultures used for analyses shown in Figs. 2–5. B Representative images of immunofluorescence staining of day 14 co-cultures using the indicated primary antibodies and α-BTX and DAPI. Scale bar for all panels: 50 μm. C The graphs report quantitative analysis of the percentage of α-BTX positive fibers (left) and TUJ1 fluorescence intensity (right), at day 14 and 28 of co-culture. Each dot represents a replicate, consisting of an individual batch of differentiated iPSCs. For each replicate, the following number of randomly selected fields were used for the α-BTX analysis: 6 (day 14 all genotypes, replicates 1 and 2; day 28 FUSWT, replicate 1; day 28 FUSWT + HuD, replicate 3); 5 (day 14 all genotypes, replicate 3; day 28 FUSWT, replicate 3; day 28 FUSP525L, all replicates) or 4 (day 28 FUSWT, replicate 2; day 28 FUSWT + HuD, replicates 1 and 2). 6 randomly selected fields were used for each replicate of the TUJ1 analysis. Error bars indicate standard deviation calculated on the average value of the replicates. Ordinary two-way ANOVA: for α-BTX positive fibers, p = 0.0715 between day 14 and 28, p = 9.7 × 10−10 among genotypes; for TUJ1 fluorescence intensity, p = 1.3 × 10−6 between day 14 and 28, p = 9.7 × 10−7 among genotypes; relevant post hoc Tukey test p values for multiple comparisons among genotypes within each time point are indicated in the graph. Source data are provided as a Source Data file.
