Fig. 1: A temporally resolved single cell atlas reveals the dynamic transcriptional landscape of cell populations during posterior regeneration.
From: Molecular profiles, sources and lineage restrictions of stem cells in an annelid regeneration model
a Sampling scheme illustrating posterior amputation and sampling timepoints, ranging from 0 hours post amputation (0 hpa, equivalent to a regular trunk segment) to 72 hpa, matching morphologically defined stages (st. 0 to 3). b UMAP visualization of cells, annotated by tentative cell type / population identity. c UMAP visualization showing the regenerative timepoint at which cells were sampled. d, e UMAP visualizations showing the expression of posterior identity markers (cdx, foxa) on the merged dataset, contrasting the freshly amputated sample (left) with the post-amputation time points (right) (f–i) similar UMAP visualizations, highlighting the changes in expression of stem-cell related genes (hox3, piwi, myc) (f–h) as well as the emergence of hypertranscriptomic cells (UMIs/cell) (i). j UMAP visualization of CytoTRACE values (calculated per cluster; high level indicates high differentiation potential); data for analysis provided as a Source Data file.
