Fig. 3: Misalignment of α chains reduces the number of salt bridges.

Number of salt bridges observed in the proposed alignment shown in Supplementary Fig. 1 (red) and when the middle chain (purple) or trailing chain (cyan) are misaligned with a four-residue offset A. The box and whiskers plot in B show the aggregate loss in the number of salt bridges upon misalignment. The end limits of the error bars show the minimum and maximum number of salt-bridges, the box represents the lower and upper quartile of the total number of salt bridges and the horizontal line in the box denotes the median value.