Fig. 2: Transcriptome and proteome changes in insulin-resistant kidney cell lines. | Nature Communications

Fig. 2: Transcriptome and proteome changes in insulin-resistant kidney cell lines.

From: Profiling of insulin-resistant kidney models and human biopsies reveals common and cell-type-specific mechanisms underpinning Diabetic Kidney Disease

Fig. 2

RNA and protein were simultaneously isolated from podocytes (Pods), glomerular endothelial cells (GECs), mesangial cells (MCs) and proximal tubular cells (PTCs) under basal and insulin-resistant conditions, with and without additional IR-transduction (n = 5 biological repeats/condition). a Principal component analysis (PCA) of >18,000 transcripts identified by RNAseq and >6000 proteins, detected across all cell lines and conditions studied, demonstrating the primary clustering of samples by cell type. b The number of differentially expressed (DE) transcripts and DE proteins (FDR < 0.05) within each cell type (both stable insulin receptor-expressing and non-insulin receptor transfected cells) in diabetic (insulin-resistant) vs. basal (insulin-sensitive) conditions. c Transcripts and d proteins differentially expressed IR-transduced cell lines in diabetic (insulin-resistant) vs. basal (insulin-sensitive) conditions, with examples of significantly regulated molecules highlighted (FDR < 0.05); differential expression analyses of full transcriptomics and proteomics datasets are available in the ‘Source data’ file. e Venn diagrams demonstrating the overlap of DE (FDR < 0.05, Log2FC < 0 or Log2FC > 0) transcripts or proteins between individual cell types, (analysis on IR-expressing cells alone).

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