Fig. 7: Insulin-resistant kidney cells have differential, protein-level regulation of mitochondrial dynamics. | Nature Communications

Fig. 7: Insulin-resistant kidney cells have differential, protein-level regulation of mitochondrial dynamics.

From: Profiling of insulin-resistant kidney models and human biopsies reveals common and cell-type-specific mechanisms underpinning Diabetic Kidney Disease

Fig. 7

a Normalised enrichment scores (NES) for enriched mitochondrial gene signatures in insulin-resistant cell lines, highlighting predominant regulation at the protein-level (*q < 0.1, **q < 0.05, ***q < 0.01). b Number of significantly up- or down-regulated (FDR < 0.1) mitochondrial proteins (based on having mitochondrial GOCC annotation) detected in our proteomics datasets. c Heatmap of log2 Fold Change (insulin resistant vs. basal) for the respiratory chain complex transcripts and proteins detected in all four cell types *FDR < 0.1, **FDR < 0.05, ***FDR < 0.01. d Heatmap of log2 Fold Change (insulin resistant vs basal) for TCA cycle transcripts and proteins detected in all four cell types *FDR < 0.1, **FDR < 0.05, ***FDR < 0.01. e Heatmap of log2 Fold Change (insulin resistant vs. basal) for glycolysis transcripts and proteins detected in all 4 cell types *FDR < 0.1, **FDR < 0.05, ***FDR < 0.01. f Schematic diagram of mitochondrial bioenergetic processes likely dysregulated in Pods, MCs and PTCs based on proteomics data (upregulated = red, downregulated = blue), created in BioRender. Sinton, M. (2023) BioRender.com/s01v107. g qPCR results of MT-COXII mRNA (n = 5, each cell type and condition, two-tailed t-test, data are presented as mean values ± SEM) and h densitometry values for COX-II protein expression (n = 4 podocyte, n = 5 glomerular endothelial cells, n = 5 mesangial cells, n = 3 proximal tubular cells, two-tailed t-test, data are presented as mean values ± SEM), i qPCR results of NDUFB8 mRNA (n = 5, each cell type and condition, two-tailed t-test) and j densitometry values for NDUFB8 protein expression (n = 3 podocyte, n = 4 glomerular endothelial cells, n = 4 mesangial cells, n = 3 proximal tubular cells, two-tailed t-test).

Back to article page