Fig. 8: Mitochondrial metabolism is differentially impaired in insulin-resistant kidney cells. | Nature Communications

Fig. 8: Mitochondrial metabolism is differentially impaired in insulin-resistant kidney cells.

From: Profiling of insulin-resistant kidney models and human biopsies reveals common and cell-type-specific mechanisms underpinning Diabetic Kidney Disease

Fig. 8

a Seahorse extracellular flux analysis of oxygen consumption rate (OCR) in ‘Basal’ or ‘Insulin resistant’ podocytes, glomerular endothelial cells (GEC), mesangial cells (MCs) and proximal tubular cells (PTCs) following injections of oligomycin (1.5 µM), FCCP (1 µM) and antimycin A (0.5 µM) plus rotenone (0.5 µM) at the indicated time points. b, c Percentage of ATP production attributed to b mitochondrial respiration or c glycolysis in each cell line under ‘Basal’ or ‘Insulin Resistant’ conditions in podocytes (‘Pod’, n = 6 ‘Basal’ vs. n = 7 ‘Insulin Resistant’), glomerular endothelial cells (‘GEC’, n = 6 ‘Basal’ vs. n = 5 ‘Insulin Resistant’), mesangial cells (‘MC’, n = 7 ‘Basal’ vs. n = 6 ‘Insulin Resistant’) and proximal tubular cells (PTC, n = 4 ‘Basal’ vs. n = 4 ‘Insulin Resistant’) two-tailed t-test, data are presented as mean values ± SEM; d OCR values per minute, per 1000 cells representing maximal respiratory capacity (in ‘Insulin Resistant’ vs. ‘Basal’ conditions in Podocytes (‘Pod’, n = 6 ‘Basal’ vs. n = 7 ‘Insulin Resistant’), glomerular endothelial cells (‘GEC’, n = 6 ‘Basal’ vs. n = 5 ‘Insulin Resistant’), mesangial cells (‘MC’, n = 7 ‘Basal’ vs. n = 6 ‘Insulin Resistant’) and proximal tubular cells (PTC, n = 4 ‘Basal’ vs. n = 4 ‘Insulin Resistant’) two-tailed t-test, data are presented as mean values ± SEM; e OCR values per minute, per 1000 cells representing mitochondrial spare respiratory capacity (in ‘Insulin Resistant’ vs. ‘Basal’ conditions in podocytes (‘Pod’, n = 6 ‘Basal’ vs. n = 7 ‘Insulin Resistant’), glomerular endothelial cells (‘GEC’, n = 6 ‘Basal’ vs. n = 5 ‘Insulin Resistant’), mesangial cells (‘MC’, n = 7 ‘Basal’ vs. n = 6 ‘Insulin Resistant’) and proximal tubular cells (PTC, n = 4 ‘Basal’ vs. n = 4 ‘Insulin Resistant’) two-tailed t-test, data are presented as mean values ± SEM.

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