Fig. 2: Anaeramoeba symbionts are housed in a membrane network with connections to the plasma membrane.
From: A unique symbiosome in an anaerobic single-celled eukaryote
a–c FIB-SEM slice of A. flamelloides BUSSELTON2, showing (b) segmented regions of interest (symbiont–blue, hydrogenosome–red, symbiosome membrane–gold, nucleus–purple, granules–aubergine, other prokaryotes– blue green, plasma membrane–yellow). c overlay of (a, b). d–f Rendered volume of FIB-SEM slices showing segmented regions of interest. d Symbiont, symbiosome membrane, nucleus and plasma membrane. e Hydrogenosomes, nucleus and plasma membrane. f Overlay of all regions of interest in (d, e), and microtubules (MTs) (symbiont–blue, hydrogenosome–red, symbiosome membrane–gold, nucleus–purple, plasma membrane– yellow, MTs–pink). g A clipped 3D rendering of A. flamelloides BUSSELTON2 symbionts (blue), hydrogenosomes (red), symbiosome membrane (gold) and nucleus (purple) showing the internal structure of the symbiosome. h–i Two different views of 3D surface rendering of the components of the symbiosome connected to the plasma membrane (light blue). Plasma membrane (yellow) and nucleus (purple). Symbiosome connections to the plasma membrane are indicated by black arrows. Scale bar (a–c), 1 µm.
