Fig. 6: Isomer-specific profiling of the murine N-glycome.
From: Non-targeted N-glycome profiling reveals multiple layers of organ-specific diversity in mice
Closely related structural N-glycan isomers were separated with PGC-LC. N-glycan retention libraries in conjunctions with MS/MS data were used to identify the exact structure of the respective isomers. All retention times were normalized to the Man5 N-glycan. A Elution profiles for the compositions Hex5HexNAc4Fuc1Neu5Ac2 (purple), Hex5HexNAc4Fuc1Neu5Ac1Neu5Gc1 (red), and ex5HexNAc4Fuc1Neu5Gc2 (light blue) across selected tissues. B Elution profiles for the composition Hex3HexNAc5Fuc1 across kidney (red), brain (blue), heart (green), pancreas (orange), liver (grey), and spleen (purple). C Elution profiles of the heavily fucosylated composition Hex5HexNAc4Fuc3 in the kidney (red), brain (blue), seminal vesicle (green), and pancreas (orange). D Biosynthetic pathway leading to complex-type and hybrid-type N-glycans and elution profiles of the composition Hex5HexNAc4Fuc1 in the brain (red), heart (blue), lung (green), pancreas (orange), seminal vesicles (grey), and kidney (purple).
