Fig. 5: Reanalysis of publicly available spatial- and untargeted lipidomics data for mouse eye tissues.

a A sunburst plot summarizing species/tissue-specific lipid database statistics containing collision-cross section (CCS) values. An eye-lipidome table with m/z and CCS values for 525 unique lipids was used to annotate lipids in MSI data analysis. A summary table of peak annotations in the analyzed MSI data is also provided. The abbreviations of FA, GL, GP, PR, SP, and ST mean fatty acyls, glycerolipids, glycerophospholipids, prenol lipids, sphingolipids, and sterol lipids, respectively. The colors in the sunburst plots were automatically generated and do not have any specific meaning. b Hematoxylin and eosin (HE) staining and MSI data in eye tissues from Acsl6^+/- and Acsl6^-/- mice. Ion distributions for five lipid molecules are shown. The reference m/z and CCS values for each lipid molecule are listed, with EAD-MS/MS-based annotations for each precursor m/z value in parentheses (n = 2 biologically independent samples available at the public repository). c Reanalysis of publicly available untargeted lipidomics data examining eye tissues from Acsl6^+/- and Acsl6^-/- mice at 10 weeks and 2 years of age (n = 3 biologically independent samples). Here, “22:6” denotes DHA, while “28:6,” “30:5,” “32:4,” “32:5,” “32:6,” “34:4,” “34:5,” “34:6,” “36:6,” and “38:6” are defined as VLC-PUFAs. An asterisk indicates acyl chains other than DHA and VLC-PUFA, with the sum of lipid molecules labeled ‘*_DHA’ or ‘*_VLC-PUFA’. ‘Other PCs’ refers to the total abundance of PC molecules not containing DHA or VLC-PUFA). Source data are provided as a Source Data file.