Fig. 6: PABPCs and DDX6/LSM14B counteract each other on the same mRNA.
From: Two mechanisms repress cyclin B1 translation to maintain prophase arrest in mouse oocytes
a, c, e Representative fluorescence images of mouse GV oocytes expressing mClover3 with different numbers of PP7s and different lengths of poly(A) tails, mScarlet, and tdPCP, tdPCP-Ddx6 or tdPCP-Lsm14b at 8 h after mRNA injection. Schematic diagrams of the experiments are shown on the left. Green, mClover3; magenta, mScarlet. b, d, f Quantification and normalization of the fluorescence intensity ratio of mClover3 to mScarlet in (a, c, e). Data are all from two independent experiments. The number of analyzed oocytes is specified in italics. Data are shown as mean ± SD. P-values were calculated using one-way ANOVA with Tukey’s post-hoc test. P values are designated as ****P < 0.0001. Scale bars, 10 µm. Source data are provided as a Source Data file.
