Fig. 2: Adhibin causes cell detachment, prevents cell spreading, and inhibits migration.

A Representative z-projections (scale bar: 20 μm) and z-to-y axis views (scale bar: 1 μm) of control, 1% DMSO, and 25 μM adhibin treated MLE-12 cells stained for actin. B Cell area (two-tailed unpaired t-test, two-tailed Mann–Whitney test for two-column comparison, or one-way Kruskal–Wallis test for multiple comparisons), (C) height (two-tailed Mann–Whitney test for two-column comparison, or one-way Kruskal–Wallis test for multiple comparisons), (D) height-area correlation, and (E) circularity (two-tailed unpaired t-test, or one-way ANOVA for multiple comparisons) of control and adhibin treated MLE-12 cells. F Representative z-stack projections (scale bar: 20 μm) and z-to-y stack (scale bar: 1 μm) of control, 1% DMSO, and 25 μM adhibin treated A549 cells stained for actin. G Cell area (two-tailed Mann–Whitney test for two-column comparison, or one-way Kruskal–Wallis test for multiple comparisons) and (H) height (two-tailed unpaired t-test, or one-way ANOVA for multiple comparisons) of control and adhibin-treated A549 cells. I Spreading of B16-F1 cells, control and adhibin treated. Scale bar: 10 μm. J Radar plots of single B16-F1 cells in the absence and presence of adhibin. K Cell area of B16-F1 cells upon adhibin treatment over time. L Migration speed (two-tailed Mann–Whitney test or unpaired t-test for two-column comparison), (M) mean square displacement, and (N) directionality index (two-tailed Mann–Whitney test or unpaired t-test for two-column comparison) of control, 1% DMSO and adhibin treated B16-F1 cells. n is the number of cells analysed. All data are represented as mean ± S.D.