Fig. 4: Adhibin causes Myo9b misdistribution and interferes with RhoA-signalling. | Nature Communications

Fig. 4: Adhibin causes Myo9b misdistribution and interferes with RhoA-signalling.

From: An allosteric inhibitor of RhoGAP class-IX myosins suppresses the metastatic features of cancer cells

Fig. 4

A Representative z-projections showing actin and active RhoA (colour-coded dtomato-rothekin intensity, scale bars: 20 μm) and (B) Myo9b and actin localization in adhibin-treated B16-F1 cells (scale bars: 10 μm). C z-Projections showing localization of Myo9b in B16-F1 cells before and after adhibin treatment. The red line defines the distance from rear to front. Scale bars: 20 μm. D Intensity profile of Myo9b along the rear to front section of B16-F1 cells in the presence and absence of adhibin. E pMLC2 and actin localization in 1% DMSO and 5 μM adhibin treated B16-F1 cells. Scale bars: 10 μm. F z-Projections of native and Myo9b-eGFP-overexpressing B16-F1 cells showing Myo9b and actin localization. Overexpression of myo9b affects lamellipodium architecture and cell shape. Scale bars: 20 μm. G Representative z-projections of NIH-3T3 cells showing Myo9b localization at tips of filopodia and retraction fibres. Scale bars: 10 μm. H Percentage of Myo9b at tips of filopodia and retraction fibres in NIH-3T3 cells with and without adhibin (two-tailed unpaired t-test). n is the number of cells analysed. All data are represented as mean ± S.D. All experiments were reproduced at least three times with similar results.

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