Fig. 6: Adhibin interferes with RhoA-mediated activation of NM2 contractility. | Nature Communications

Fig. 6: Adhibin interferes with RhoA-mediated activation of NM2 contractility.

From: An allosteric inhibitor of RhoGAP class-IX myosins suppresses the metastatic features of cancer cells

Fig. 6

A, B Representative sum-projections showing actin and pMLC2 localization in adhibin-treated A459 and MLE-12 cells. Scale bars: 20 μm. C, D Amount of pMLC2 in control and adhibin-treated A459 cells, and MLE-12 cells (two-tailed Mann–Whitney test for two-column comparison, or one-way Kruskal–Wallis test for multiple comparisons). E, G Western blots comparing the amount of pMLC2 between adhibin-treated and A549 and MLE-12 cells relative to GAPDH. F, H Normalized pMLC2 intensity from three individual western blots. In all bar diagrams n is the number of experiments and in all box plots n is the number of cells analysed. All data are represented as mean ± S.D. a.u. = arbitrary units.

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