Fig. 3: Cav1 regulates adipocyte morphology and deformability.
From: Plasma membrane remodeling determines adipocyte expansion and mechanical adaptability
a Masson’s Trichrome staining of epidydimal VAT. Scale bar: 100 µm. b Average adipocyte PM-LD distance in EM images. N = 31+/+ , 13-/- adipocytes, wilcoxon test p value = 3.6 × 10−5. c Adipocyte classification based on the existence (magenta, asterisks) or absence (yellow, triangles) of surrounding collagen. d Percentage of fibrotic epidydimal VAT adipocytes of 45 weeks-old HFD animals. Wilcoxon test p value = 0.002, n = 9 animals. e Influence of surrounding fibrosis in adipocyte area. Lines join average adipocyte values for depots from the same animal. Paired t-test p values: 0.0007 for +/+ (8 animals) and 0.0012 for −/− (9 animals). f Examples of fibrotic (asterisks) and non-fibrotic adipocytes. Scale bar: 50 µm. g Adipocyte mean circularity during aging, with 95% confidence intervals. Sample size: 123+/+ , 98−/− animals. Effect of diet on adipocyte circularity (h) and area (i) in 45-week-old animals. t-test for regression coefficients p-values: effect of diet in WT circularity and area, 0.028 and 6.50 × 10−14, respectively; diet-genotype interaction, 0.009 for circularity and 0.19 for area. Sample size for h and i: For ND, 30 animals +/+ and 17 −/−; for HFD, 15 per genotype. Boxplots show first (Q1), second (median), and third (Q3) quantiles, and whiskers extend from Q1-1.5·IQR to Q3+1.5·IQR. p-value codes: *<0.05, **<0.01, ***<0.001. All tests are two-sided. Source data provided as a SourceData.zip.
