Fig. 4: Cav1 regulates adipocyte mechanical properties.
From: Plasma membrane remodeling determines adipocyte expansion and mechanical adaptability
a Schematic representation of compression assay on VAT. b LDH activity in media. Lines connect control and compressed contralateral depots of the same animal, paired t-test p values: 4.2 × 10−5, 0.0071 (comparison between contralateral depots). Pairwise t-test with Bonferroni correction p value: 0.00017 (comparison between genotypes). c LDH activity difference between stimulated and control contralateral depots. Pairwise t-test with Bonferroni correction p value = 0.039. Sample size for k and l: 10+/+ and 9−/− animals, d Schematic cross-sections of AFM-based force spectroscopy (indentation) experiments with bottom-effect corrections143. Median Young’s Modulus for VAT (e) and in vitro differentiated adipocytes (f). For n and o: pairwise t-test p values: 2.2 × 10−7 for HFD (6 animals), 0.00035 for ND (11 animals), 0.00033 for cells (22 cells from 4 animals per genotype). g Examples of adipocytes selected for AFM. h Flipper-TR FLT as a function of adipocyte area. Sample size for isosmotic: 24+/+, 21−/− hyperosmotic: 17+/+, 14−/− cells. Average FLT and area for different z of the same cell ±SEM is represented. i Examples of Flipper-TR FLT after 120 h of HF media treatment and manually segmented membranes. Scale bar: 20 microns. Boxplots show first (Q1), second (median), and third (Q3) quantiles, and whiskers extend from Q1-1.5·IQR to Q3+1.5·IQR. p-value codes: *<0.05, **<0.01, ***<0.001. All tests are two-sided. Source data provided as a SourceData.zip.
