Table 1 Enzymatic assay of SpySystem-mediated CYP73A5Δ2-28-ATR2Δ2-77 heterodimers and the free-floating CYP73A5Δ2-28 and ATR2Δ2-77 with trans-cinnamic acid

From: Tuning architectural organization of eukaryotic P450 system to boost bioproduction in Escherichia coli

Proteins

trans-Cinnamic acida

Km (μM)

kcat (min−1)

kcat/Km (M−1 min−1, × 103)

h

CYP73A5Δ2-28 & ATR2Δ2-77

68.83 ± 14.37

0.018 ± 0.002

0.26

1.49 ± 0.39

heterodimer (I)

23.35 ± 5.88

0.198 ± 0.020

8.48

1.02 ± 0.23

heterodimer (II)

125.62 ± 151.95

0.085 ± 0.035

0.68

0.78 ± 0.34

heterodimer (III)

69.51 ± 20.16

0.020 ± 0.002

0.29

1.08 ± 0.24

heterodimer (IV)

62.98 ± 15.05

0.881 ± 0.090

13.99

1.08 ± 0.15

  1. aThe Hill’s equation was used to fit the formation rate of p-coumaric acid to the concentrations of trans-cinnamic acid. h is the Hill coefficient. Data are shown as mean±SE (n = 3 independent experiments).
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