Fig. 2: SMARRT.RSV.preF vaccination elicits humoral and cellular response in mice.

a Vaccines SMARRT.RSV.preF (0.1, 1, and 10 mcg) or Ad26.RSV.preF (10¹⁰ VPs) were administered intramuscularly to female BALB/c mice. While Ad26.RSV.preF was given as a single dose, SMARRT vaccine was administered as a 2-dose homologous regimen given 4 weeks apart. Components of this figure were sourced from Open Clip Art, under a Creative Commons Attribution 3.0 Unported License; https://creativecommons.org/licenses/by/3.0/. The serum from n = 8 biologically independent animals per group was sampled on days 0, 27 and 56. b RSV.CL57 neutralizing antibody titers and c RSV.preF specific IgG antibodies in the serum of immunized animals measured at day 27 (open circles) and day 56 (open squares). Limit of detection (LoD) is represented by the dotted line. Gray lines represents paired measurements. Statistical comparisons of day 27 to day 56 humoral responses were determined with ANOVA and adjusted for multiple comparisons with Bonferroni correction of log transformed data. d RSV.F specific T-cells measured in the spleens of 10 mcg SMARRT.RSV.preF immunized animals (n = 8 biologically independent animals), at day 56, by ICS. Different subsets of IFNγ, TNFα and IL2 positive CD8⁺ and CD4⁺ T-cells gated on Live CD45⁺ cells. Gray circles represent splenocytes stimulated with RSV.F peptide pool and black circles represent corresponding medium stimulated samples. Red horizontal lines represent median response. Source data are provided as a Source Data file.