Fig. 2: Impact of CDC42 variants on anterograde transport. | Nature Communications

Fig. 2: Impact of CDC42 variants on anterograde transport.

From: Autoinflammatory patients with Golgi-trapped CDC42 exhibit intracellular trafficking defects leading to STING hyperactivation and ER stress

Fig. 2

A Immunofluorescence analyses of PC-1, ER, Golgi and nucleus localizations in 4 different healthy donor (HDs) fibroblasts, patients Y64C and R186C at 0 and 1 h. Images are representative of at least 3 independent experiments. Scale bars: 10 µm. Quantifications of the colocalizations between PC-1 and the ER (B) or the Golgi (C) at the indicated times for each cell type. D Analysis of total PC-1 fluorescence intensity following anterograde transport. The relative intensity of PC-1 expression was normalized to 100 from the mean values of the 4 HDs at time 0. In all graphs, one dot represents the mean value from about 15 cells from one independent experiment. Results are shown as means +/- SEM of at least three biological replicates and the significance levels were calculated using ordinary one-way ANOVA (***P = 0.0006, ****P < 0.0001). Source data are provided as a Source Data file.

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