Fig. 3: Impact of CDC42 variants on retrograde transport. | Nature Communications

Fig. 3: Impact of CDC42 variants on retrograde transport.

From: Autoinflammatory patients with Golgi-trapped CDC42 exhibit intracellular trafficking defects leading to STING hyperactivation and ER stress

Fig. 3

A Immunofluorescence analyses of retrograde transport assay of Cholera toxin B subunit (CtxB) in fibroblasts from the HD8 healthy donor and from the Y64C and R186C CDC42 patients at times 0 and 8 h. Co-stainings for Golgi, ER and nuclei were performed. Images are representative of at least 3 independent experiments. Scale bars: 10 µm. Quantifications of microscopy images are shown for the CtxB—Golgi (B) and CtxB—ER (C) colocalizations at the indicated times. Transport assay data from 4 healthy donors were pooled. In all graphs, one dot represents the mean value from about 15 cells from one independent experiment. Results are shown as means +/- SEM of at least three biological replicates and the significance levels were calculated using ordinary one-way ANOVA (***P < 0.007). Source data are provided as a Source Data file.

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