Fig. 6: Golgi-trapped CDC42 variants induce STING pathway activation. | Nature Communications

Fig. 6: Golgi-trapped CDC42 variants induce STING pathway activation.

From: Autoinflammatory patients with Golgi-trapped CDC42 exhibit intracellular trafficking defects leading to STING hyperactivation and ER stress

Fig. 6

A Measurement of P-IRF3 intensity in the nuclei of THP-1 cells expressing GFP-CDC42 variants. Immunocytochemistry stainings and quantifications of P-IRF3 (B) and P-STAT1 (C) intensities in healthy donors (HDs) or CDC42 patients’ fibroblasts. Scale bars: 10 µm. In all graphs, one dot represents the mean value of fluorescence intensities from about 15 cells from one independent experiment. D Flow cytometry analyses of P-STAT1 expression in THP-1 cells expressing WT or mutant CDC42. Left: examples of P-STAT1 expression profiles in cells non stimulated (turquoise) or stimulated with IFNα (red). Right: quantifications of the P-STAT1 Mean Fluorescence Intensity (MFI) in cells upon IFNα stimulation. Each dot represents the MFI from one independent experiment. For all graphs, results are shown as means +/- SEM of at least three biological replicates and the significance levels were calculated using ordinary one-way ANOVA (*P < 0.0424; **P < 0.0086; ***P = 0.0004; ****P < 0.0001). Source data are provided as a Source Data file.

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