Fig. 1: Human myeloid cell subpopulations in HGSOC omental metastases.
From: Immunotherapy that improves response to chemotherapy in high-grade serous ovarian cancer
A UMAP showing integrated myeloid cell subpopulations (n = 13) from seven patients (3 PDS and 4 NACT). B Heatmap showing top five upregulated genes per cluster calculated using the default Wilcoxon sum rank test in Seurat package and arranged by log2FC. C Differentially enriched pathways in the macrophage clusters, GSVA analysis, coloured by z transformed mean GSVA scores. D Differentially expressed genes NACT versus PDS. Wilcoxon rank sum test. E Differentially enriched pathways NACT versus PDS using GSVA and two-sided unpaired limma-moderated t test. F Violin plot of expression level of selected genes in PDS (pink) and NACT (green) in each macrophage cluster. G CD68 and STAB1 positive cells per mm2 in HGSOC omenta (PDS = 22, NACT = 58 patients). Error bars median and 95%CI. Two-sided Mann–Whitney test. H STAB1 positive cells/mm2 comparing PDS and NACT in both BRCA-wild type (n = 42) and BRCAmutated/HRD patients (n = 27). Two-sided Mann–Whitney test. I Immunohistochemistry staining for STAB1 positive cells in patient-matched omental samples pre- and post-NACT (n = 2 patients). J i-ii) STAB1 positive cells in patient-matched samples. 42 patients were included who had pre-treatment biopsy and biopsy after NACT. 36 of the pre-NACT samples were omental metastasis and 4 peritoneal biopsies, 1 cervical and 1 liver biopsies. iii and iv) Analysis of STAB1 positive cells/mm2 in the patient-matched cohort comparing pre- and post-NACT in both BRCA-wild type (n = 21 paired samples) and BRCAmutated/HRD patients (n = 13 paired samples). Error bars at median and 95%CI. Two-sided Wilcoxon matched pairs signed rank test. K Kaplan–Meier survival and PFS curves (Mantel–Cox), STAB1-high versus low levels measured by IHC (n = 58 and 56 patients). 60% was the cut-off between STAB1 high and low levels.
