Fig. 3: Human CD8 T cell subpopulations in HGSOC omental metastasis.
From: Immunotherapy that improves response to chemotherapy in high-grade serous ovarian cancer
A UMAP showing integrated CD8 cell clusters from the above seven patients. B Heatmap showing top upregulated genes per cluster. C Differentially expressed genes in CD8 T cells NACT versus PDS. Wilcoxon rank sum test. D Differentially enriched pathways in NACT versus PDS in CD8 cells. E Percentage of CD8 cells of total CD45 cells in 31 human omental samples (n = 8 PDS, n = 23 NACT) using flow cytometry. Gating on singlet/live/CD45 + /CD3 + /CD8 cells. Error bars at median and 95%CI. Two-sided Welch t-test. F UMAP showing the integrated NKTgd cell clusters from 7 patients, 2780 and 2026 cells were annotated as NK and Tgd cells, respectively. G Heatmap showing top five upregulated genes per cluster in NKTgd subpopulations. H Differentially expressed genes in NKTgd cells in NACT versus PDS. Wilcoxon rank sum test.
