Fig. 2: Construction of IU pathway-dependent strain.
From: Yeast metabolism adaptation for efficient terpenoids synthesis via isopentenol utilization
a Schematic diagram of the construction of IUPD strains, substituting the MVA pathway with the IU pathway. b Growth of IUP2 in YPD medium supplemented with prenol using 48 deep well plates and isoprenol at various concentrations ranging from 0 to 20 g/L. c Comparison of the growth of different strains. Prenol (5 g/L) and MVA (20 mM) were added to the YPD medium as required. Data represent mean value with SD (three independent biological replicates). Statistical significance was evaluated by a two-tailed student’s t test (ns represents no significant difference, *p < 0.05, **p < 0.01, ***p < 0.001). Source data are provided as a Source Data file.
