Fig. 1: Mesoscale functional imaging revealed patchy spatial patterns of spontaneous activity throughout the marmoset neocortex.

a Schematics of large FOV calcium imaging using a primate-optimized GCaMP-expression system. Grid-based injection allows it to cover large surface areas in an occipito-parietal region (top) and a temporal region (bottom). b In vivo GCaMP fluorescence in the occipital parietal FOV. Approximate positions of cortical areas are based on the marmoset brain atlas⁷⁵. Experiments were repeated two times and similar results were obtained. Scale bar, 1 mm. c Orientation map of the occipital parietal FOV obtained with in vivo widefield calcium imaging. Positions of V1, V2, and DM are determined based on the structure of the orientation map (note the stripes in V2). Experiments were repeated two times and similar results were obtained. Scale bar, 1 mm. d A snapshot of spontaneous activity in the occipital parietal FOV. A spatial high-pass filter is applied. See Supplementary Movie 1 for non-filtered data. Experiments were repeated two times and similar results were obtained. Scale bar, 1 mm. e In vivo GCaMP fluorescence in the temporal FOV. Experiments were repeated four times and similar results were obtained. Scale bar, 1 mm. f Direction map in the temporal FOV. Direction columns indicate the position of MT. 1 mm. g A snapshot of spontaneous activity in the temporal FOV. See Supplementary Movie 2 for non-filtered data.