Fig. 5: Proliferation and invasion for different melanoma lines using knockdown siRNA. | Nature Communications

Fig. 5: Proliferation and invasion for different melanoma lines using knockdown siRNA.

From: Rab7a is an enhancer of TPC2 activity regulating melanoma progression through modulation of the GSK3β/β-Catenin/MITF-axis

Fig. 5

Proliferation monitored over 72 h using Ctb assay of the following melanoma lines: SK-MEL-5, SK-MEL-29, SK-MEL-19, UACC-62, SK-MEL-103, SK-MEL-147, and A375 in non-silencing (NS) control cells compared to TPC2 KD (n = 3–10) (a) and Rab7a KD (n = 3–10) (b). Statistical analysis of cell invasiveness determined in the lines mentioned above using transwell boyden chambers coated with matrigel in both Rab7a KO (n = 26–64) (c) and TPC2 (n = 21–58) (e). Representative images of invasion phenotype in TPC2 KD (d) and Rab7a KD (f) cells in several melanoma lines compared to NS control. Statistical significance was determined using two-way ANOVA followed by Bonferroni multiple comparisons test (a, b; n = 3, each) and by two-tailed unpaired t-test (c, e). Shown are mean values ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. All scale bars = 25 µm. All n numbers represent biological replicates. Source data are provided as Source Data file.

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