Fig. 3: LADs reorganization is linked to aging-associated genes in MAD-MSCs. | Nature Communications

Fig. 3: LADs reorganization is linked to aging-associated genes in MAD-MSCs.

From: Disorganized chromatin hierarchy and stem cell aging in a male patient of atypical laminopathy-based progeria mandibuloacral dysplasia type A

Fig. 3

a Representative distribution of LADs at specific genome loci in WT and MAD-MSCs, including A-LADs and B-LADs. b The length of genomic coverage of A-LADs and B-LADs in WT and MAD-MSCs. c Boxplot showing Lamin enrichment in WT A-LADs (n = 340), MAD A-LADs (n = 535), WT B-LADs (n = 257), and MAD B-LADs (n = 326), with 2 biological replicates for lamins ChIP-seq. Box plots display the median as the center line, the 25th and 75th percentiles as the bounds of the box, and the whiskers represent the minimum and maximum values within 1.5 times the interquartile range from the lower and upper quartiles. All p-values were determined using the two-sided Wilcoxon rank-sum test. d, e Heatmap of differential A-LADs and B-LADs in WT and MAD-MSCs. f Differentially expressed genes associated with LADs reorganization. Genes with over 2-fold transcriptional changes were counted. g Cross-analysis of the enrichment of dysregulated genes due to LAD reorganization in MAD-MSCs with geroprotection/senescence-associated profile in different hMSCs aging models. Color depth indicates enrichment score.

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