Fig. 1: Synergism of cortex-wide single-cell resolution calcium imaging and electrical stimulation. | Nature Communications

Fig. 1: Synergism of cortex-wide single-cell resolution calcium imaging and electrical stimulation.

From: Mesoscale neuronal granular trial variability in vivo illustrated by nonlinear recurrent network in silico

Fig. 1

a Schematic drawing of the experiment setup by combining RUSH imaging system and deep brain stimulus (DBS) recording. b Multimodal signals detected in the head-fixed mice include the synchronous changes of pupil area, running speed, and calcium traces of neurons. The stimulus electrode would deliver a biphasic waveform to Cla several times. c Example of cortex-wide calcium imaging at single-cell level obtained by RUSH system. Enlarged views of the cortical areas marked by the boxes show clear cellular profiles of individual neurons. This neuron distribution result is similar in other mice (n = 15 mice). d Approximately 40,000 active neurons across wide cortical regions were detected in a typical mouse. e Distributions of neurons in 12 cortical regions (dots are the percentage of neuron number in the corresponding area from individual mice, n = 15 mice). f Correlation analysis of the cortex-wide activities at the single-cell level. Source data are provided as a Source Data file.

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