Fig. 2: Accelerated locomotion increases histamine release in the SUB but not EC.
From: Histamine-tuned subicular circuit mediates alert-driven accelerated locomotion in mice
Schematic diagram and representative image of viral injection of AAV-hSyn-HA1h into the SUB (a) or EC (c) of WT mice. Scale bar = 200 µm. The representative speed (upper) and corresponding fluorescence signal of histamine concentration changes (bottom) in SUB (b) or EC (d) during locomotion in the open field test. Fluorescence signal, heatmap, corresponding speed, and quantification of the mean fluorescence values of histamine concentration changes in SUB (e, f) or EC (g, h) during locomotor acceleration. n = 14 mice, ***p = 0.0001 for (f), n = 18 mice, p = 0.7850 for (h). Representative fluorescence signal, heatmaps, and quantification of mean fluorescence values of histamine concentration changes in SUB (i, j) or EC (k, l) during the rotarod test (j: n = 12 mice, p = 0.5537 B.S vs. 5 rpm, **p = 0.0016 B.S vs. 6 rpm, ***p = 0.0002 B.S vs. 7 rpm, *p = 0.0187 B.S vs. 8 rpm; l: n = 18 mice, p = 0.8322 B.S vs. 5 rpm, p = 0.4192 B.S vs. 6 rpm, p = 0.6334 B.S vs. 7 rpm, p = 0.5721 B.S vs. 8 rpm). Representative fluorescence signal, heatmap, and quantification of mean fluorescence values of histamine concentration changes in SUB (m, n) or EC (o, p) during treadmill test (n: n = 16 mice, **p = 0.0046 Acc vs. B.S, p = 0.0720 Max vs. Acc, p = 0.0855 Dec vs. Max; p: n = 18 mice, p = 0.2022 Acc vs. B.S, p = 0.1775 Max vs. Acc, p = 0.6324 Dec vs. Max). Two-tailed Student’s t test for (f, h, n, p) and one-way ANOVA followed by the Dunnett post hoc test for (j, l). Schematics in a, c were drew by PowerPoint. Bar graphs represent mean ± SEM. Source data are provided as a Source Data file.
