Fig. 5: Chemogenetic inhibition of SUB glutamatergic neuron blocks pro-locomotion effect of TMN-SUB histaminergic circuit.
From: Histamine-tuned subicular circuit mediates alert-driven accelerated locomotion in mice
a Schematic and image of injection of AAV-CaMKIIα-hM3D-mCherry into SUB of WT mice, scale bar = 200 μm. b CaMKIIα-hM3D-based glutamatergic neuron excitation in vivo resulted in c-fos expression in SUB. n = 6 mice. Effects of chemogenetic activation of SUB glutamatergic neurons on the distance traveled (c), and average speed (d) in open field test, n = 7 mice (c: p = 0.05; d: *p = 0.0496). e Representative velocity distribution of saline control group and the CNO group. Total time (f), and distance moved (g) in open field test in 5-speed subsections of saline control group and CNO group. n = 7 mice (f: *p = 0.0100, *p = 0.0101, *p = 0.0381, p = 0.3358, *p = 0.0362; g: p = 0.1169, p = 0.0506, p = 0.7248, *p = 0.0116, *p = 0.0349). h Schematic diagram and representative image of viral injection of AAV-CAG-FLEX-oChIEF-tdTomato into the TMN, and AAV-CaMKIIα-hM4D-GFP into the SUB of HDC-CreERT2 mice, and the timeline schematic diagram. scale bar = 200 μm. Effects of optogenetic activation of TMN-SUB circuit terminals combined with chemogenetic inhibition of SUB glutamatergic neurons on distance traveled (i), average speed (j) in the open field test. Saline group, n = 10 mice; CNO group, n = 10 mice; saline + 470 nm group, n = 11 mice; CNO + 470 nm group, n = 12 mice (i: **p = 0.0060 saline + 470 nm vs. saline group, ####p < 0.0001 CNO + 470 nm vs. saline + 470 nm group; j: **p = 0.0060 saline + 470 nm vs. saline group, ####p < 0.0001 CNO + 470 nm vs. saline + 470 nm group). Two-tailed Student’s t test for (c, d, f, g) and one-way ANOVA followed by the Turkey’s post hoc test for (i, j). Schematics in a, h were drew by PowerPoint. Bar graphs represent mean ± SEM. Source data are provided as a Source Data file.
