Fig. 2: An anorectic pathway between GFRAL neurons and the parabrachial nucleus. | Nature Communications

Fig. 2: An anorectic pathway between GFRAL neurons and the parabrachial nucleus.

From: Brainstem BDNF neurons are downstream of GFRAL/GLP1R signalling

Fig. 2

a Schematic illustrating fibre optic implant above the elPBN of GfralCre:ChR2-eYFP mice and wild-type littermates, GfralWT:ChR2-eYFP. Based on the Paxinos and Franklin Mouse Brain Atlas56. b Dual-fluorescence labelling showing FOS expression (magenta) close to green (eYFP) fibres of GfralCre:ChR2-eYFP mice (bottom) and GfralWT:ChR2-eYFP (top) following 10 Hz optogenetic stimulation of GFRAL terminals in the elPBN. c Cumulative food intake in overnight fasted GfralCre:ChR2-eYFP (red squares; n = 6) and GfralWT:ChR2-eYFP (black squares; n = 8) mice during 10 Hz optogenetic stimulation for 60 min in the elPBN (**p = 0.0019; two-way ANOVA followed by Sidak’s multiple comparisons test). AP (area postrema), elPBN (exterolateral parabrachial nucleus), scp (superior cerebellar peduncle). All data presented as mean ± SEM. Scale bars indicate 100 μm. Source data are provided as a Source Data file.

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